Sexual Reproduction in Plants

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Sexual Reproduction

In previous classes reproduction in lower plants like algae and bryophytes was discussed in detail. Sexual reproduction involves the production and fusion of male and female gametes. The former is called gametogenesis and the latter is the process of fertilization. Let us recall the sexual reproduction in algae and bryophytes.

They reproduce by the production of gametes which may be motile or non motile depending upon the species. The gametic fusion is of three types (Isogamy, Anisogamy and Oogamy). In algae external fertilization takes place whereas in higher plants internal fertilization occurs.

Flower

A flower is viewed in multidimensional perspectives from time immemorial. It is an inspirational tool for the poets. It is a decorative material for all the celebrations. In Tamil literature the fie lands are denoted by different flowers. The flags of some countries are embedded with flowers. Flowers are used in the preparation of perfumes.

For a Morphologist, a flower is a highly condensed shoot meant for reproduction. As you have already learned about the parts of a flower in Unit II of Class XI, let us recall the parts of a flower. A Flower possesses four whorls – Calyx, Corolla, Androecium and Gynoecium.

Androecium and Gynoecium are essential organs (Figure 1.3). The process or changes involved in sexual reproduction of higher plants include three stages. They are Prefertilization, Fertilization and Post fertilization changes. Let us discuss these events in detail.
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Vegetative Propagation – Definition, Types, Examples & Explanations

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Vegetative Propagation – Definition, Types, Examples & Explanations

Natural methods

Natural vegetative reproduction is a form of asexual reproduction in which a bud grows and develops into a new plant. The buds may be formed in organs such as root, stem and leaf. At some stage, the new plant gets detached from the parent plant and starts to develop into a new plant.

Some of the organs involved in the vegetative reproduction also serve as the organs of storage and perennation. The unit of reproductive structure used in propagation is called reproductive propagules or diaspores. Some of the organs that help in vegetative reproduction are given in Figure 1.1.
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A. Vegetative reproduction in root

The roots of some plants develop vegetative or adventitious buds on them. Example Murraya, Dalbergia and Millingtonia. Some tuberous adventitious roots apart from developing buds also store food. Example Ipomoea batatus and Dahlia. Roots possessing buds become detached from the parent plant and grow into independent plant under suitable condition.

B. Vegetative reproduction in stem

From the Unit 3 of class XI (Vegetative morphology) you are familiar with the structure of various underground stem and sub aerial stem modifiations. Thse include rhizome (Musa paradisiaca, Zingiber offinale and Curcuma longa); corm (Amorphophallus and Colocasia); tuber (Solanum tuberosum); bulb (Allium cepa and Lilium) runner (Centella asiatica); stolon (Mentha, and Fragaria); offet (Pistia, and
Eichhornia); sucker (Chrysanthemum) and bulbils (Dioscorea and Agave). The axillary buds from the nodes of rhizome and eyes of tuber give rise to new plants.

C. Vegetative reproduction in leaf

In some plants adventitious buds are developed on their leaves. When they are detached from the parent plant they grow into new individual plants. Examples: Bryophyllum, Scilla, and Begonia. In Bryophyllum, the leaf is succulent and notched on its margin.

Adventious buds develop at these notches and are called epiphyllous buds. Thy develop into new plants forming a root system and become independent plants when the leaf gets decayed. Scilla is a bulbous plant and grows in sandy soils. The foliage leaves are long and narrow and epiphyllous buds develop at their tips. Thse buds develop into new plants when they touch the soil.

Advantages of natural vegetative reproduction

  • Only one parent is required for propagation.
  • The new individual plants produced are genetically identical.
  • In some plants, this enables to spread rapidly. Example: Spinifex
  • Horticulturists and farmers utilize these organs of natural vegetative reproduction for cultivation and to harvest plants in large scale.

Disadvantage of natural vegetative reproduction

New plants produced have no genetic variation.

Artificial Methods

Apart from the above mentioned natural methods of vegetative reproduction, a number of methods are used in agriculture and horticulture to propagate plants from their parts. Such methods are said to be artifiial propagation.

Some of the artifiial propagation methods have been used by man for a long time and are called conventional methods. Now-a-days, technology is being used for propagation to produce large number of plants in a short period of time. Such methods are said to be modern methods.

A. Conventional methods

The common methods of conventional propagation are cutting, grafting and layering.

a. Cutting:

It is the method of producing a new plant by cutting the plant parts such as root, stem and leaf from the parent plant. The cut part is placed in a suitable medium for growth. It produces root and grows into a new plant.

Depending upon the part used it is called as root cutting (Malus), stem cutting (Hibiscus, Bougainvillea and Moringa) and leaf cutting (Begonia, Bryophyllum). Stem cutting is widely used for propagation.

b. Graftng:

In this, parts of two different plants are joined so that they continue to grow as one plant. Of the two plants, the plant which is in contact with the soil is called stock and the plant used for graftng is called scion (Figure 1.2 a). Examples are Citrus, Mango and Apple.

There are different types of graftng based on the method of uniting the scion and stock. Thy are bud graftng, approach graftng, tongue graftng, crown graftng and wedge graftng.
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(i) Bud graftng:

A T – shaped incision is made in the stock and the bark is lifted. The scion bud with little wood is placed in the incision beneath the bark and properly bandaged with a tape.

(ii) Approach graftng:

In this method both the scion and stock remain rooted. The stock is grown in a pot and it is brought close to the scion. Both of them should have the same thickness. A small slice is cut from both and the cut surfaces are brought near and tied together and held by a tape. After 1-4 weeks the tip of the stock and base of the scion are cut of and detached and grown in a separate pot.

(iii) Tongue grafting:

A scion and stock having the same thickness is cut obliquely and the scion is fi into the stock and bound with a tape.

(iv) Crown grafting:

When the stock is large in size scions are cut into wedge shape and are inserted on the slits or clefts of the stock and fixed in position using graft wax.

(v) Wedge grafting:

In this method a slit is made in the stock or the bark is cut. A twig of scion is inserted and tightly bound so that the cambium of the two is joined.

c. Layering:

In this method, the stem of a parent plant is allowed to develop roots while still intact. When the root develops, the rooted part is cut and planted to grow as a new plant. Examples: Ixora and Jasminum. Mound layering and Air layering are few types of layering (Figure 1.2 b).
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i. Mound layering:

The method is applied for the plants having flxible branches. The lower branch with leaves is bent to the ground and part of the stem is buried in the soil and tip of the branch is exposed above the soil. After the roots emerge from the part of the stem buried in the soil, a cut is made in parent plant so that
the buried part grow into a new plant.

ii. Air layering:

In this method the stem is girdled at nodal region and hormones are applied to this region which promotes rooting. The portion is covered with damp or moist soil using a polythene sheet. Roots emerge in these branches after 2-4 months. Such branches are removed from the parent plant and grown in a
separate pot or ground.

Advantages of conventional methods

  • The plants produced are genetically uniform.
  • Many plants can be produced quickly by this method.
  • Some plants produce little or no seeds; in others, the seeds produced do not germinate. In such cases, plants can be produced in a short period by this method.
  • Some plants can be propagated more economically by vegetative propagation. Example: Solanum tuberosum.
  • Two diffrent plants with desirable characters such as disease resistance and high yield can be grafted and grown as a new plant with the same desirable characters.

Disadvantages of conventional methods

  • Use of virus infected plants as parents produces viral infected new plants.
  • Vegetative structures used for propagation are bulky and so they are diffilt to handle and store.

Asexual Reproduction in Plants

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Asexual Reproduction

The reproduction method which helps to perpetuate its own species without the involvement of gametes is referred to as asexual reproduction we know that reproduction is one of the attributes of living things and the diffrent types of reproduction have also been discussed.

Lower plants, fungi and animals show diffrent methods of asexual reproduction. Some of the methods include, formation of Conidia (Aspergillus and Penicillium); Budding (Yeast and Hydra); Fragmentation (Spirogyra); production of Gemma (Marchantia); Regeneration (Planaria) and Binary fision (Bacteria).

The individuals formed by this method is morphologically and genetically identical and are called clones.

Higher plants also reproduce asexually by diffrent methods which are given below:

Asexual reproduction is a type of reproduction that does not involve the fusion of gametes or change in the number of chromosomes. The offspring that arise by asexual reproduction from either unicellular or multicellular organisms inherit the full set of genes of their single parent.

In asexual reproduction, an individual can reproduce without involvement with another individual of that species. The division of a bacterial cell into two daughter cells is an example of asexual reproduction.
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7 types of Asexual Reproduction

  • Budding: A form of asexual reproduction of yeast in which a new cell grows out of the body of a parent.
  • Vegetative Reproduction: Plants budding which creates a runner hich sends a clone.
  • Parthenogenesis
  • Binary Fission.
  • Regeneration.
  • Fragmentation.
  • Spores.

Biology | Definition, History, Explanations and Examples of Biological Concepts

Learn Biology Online – Definitions, Topics and Importance of Biology

Bio Botany – Chapters with Concepts

Asexual and Sexual Reproduction in Plants

Classical Genetics

Chromosomal Basis of Inheritance

Principles and Processes of Biotechnology

Plant Tissue Culture

Principles of Ecology

Ecosystem

Environmental Issues

Plant Breeding

Economically Useful Plants and Entrepreneurial Botany

Diversity of Living World 

Plant Kingdom 

Vegatative Morphology 

Reproductive Morphology 

Taxonomy and Systematic Botany

Cell: The Unit of Life 

Cell Biology and Biomolecules 

Cell Biology and Biomolecules 

Tissue and Tissue System 

Secondary Growth 

Transport in Plants 

Mineral Nutrition 

Photosynthesis 

Respiration 

Plant Growth and Development 

Bio Zoology – Chapters with Concepts

Reproduction in Organisms

Human Reproduction

Reproductive Health

Principles of Inheritance and Variation

Molecular Genetics

Evolution

Human Health and Diseases

Microbes in Human Welfare

Applications of Biotechnology

Organisms and Population

Biodiversity and its Conservation

Environmental Issues

The Living World 

Kingdom Animalia 

Tissue Level of Organisation 

Organ and Organ Systems in Animals 

Digestion and Absorption 

Respiration

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Trends in Economic Zoology 

Techniques in Genetic Engineering

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Techniques in Genetic Engineering

There are several techniques used in recombinant DNA technology or gene manipulation. The most frequently used methods are agarose gel electrophoresis, isolation and purification of nucleic acids, nucleic acid blotting techniques, DNA sequencing, chemical synthesis of DNA, gene transfer methods, polymerase chain reaction, construction of gene library, radiolabeling of nucleic acids etc, few of them are discussed here.

Agarose Gel Electrophoresis

Electrophoresis refers to the movement of charged molecules in an electric field. The negatively charged molecules move towards the positive electrode while the positively charged molecules migrate towards the negative electrode. Gel electrophoresis is a routinely used analytical technique for the separation and purification of specific DNA fragments.

The gel is composed of either polyacrylamide or agarose. Polyacrylamide gel electrophoresis (PAGE) is used for the separation of smaller DNA fragments while agarose electrophoresis is convenient for the separation of DNA fragments ranging in size from 100 base pairs to 20 kilobase pairs.

Gel electrophoresis can also be used for the separation of RNA molecules. A diagramatic view of the agarose gel electrophoresis unit is shown in Figure 12.30 a.
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Steps

  1. Gel is set with wells on one end.
  2. The gel is placed in an electrophoresis apparatus and covered with buffer solution.
  3. The DNA samples along with tracer dye are placed in the wells of gel.
  4. Power supply is switched on and gel is run till the tracer dye reaches the end of the gel.

As the DNA is negatively charged, DNA fragments move through the gel towards the positive electrode. The rate of migration of DNA is dependent on the size and shape. In general, smaller linear fragments move faster than the larger ones.

Hence, gel electrophoresis can be conveniently used for the separation of a mixture of DNA fragments, based on their size. The bands of the DNA can be detected by soaking the gel in ethidium bromide solution (Ethidium bromide can also be added to molten agarose prior to setting the gel).

When activated by ultraviolet radiation, DNA base pairs in association with ethidium bromide, emit orange fluorescence. And in this way the DNA fragments separated in agarose electrophoresis can be identified (Figure 12.30b).
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PAGE is composed of chains of acryl amide monomers crosslinked with methylene bisacryalmide units. The pore size of the gel is dependent on the total concentration of monomers and the cross links. PAGE is used for the separation of single stranded DNA molecules that differ in length by just one nucleotide.

Agarose gels cannot be used for this purpose. This is because polyacrylamide gels have smaller pore sizes than agarose gels and allow precise separation of DNA molecule from 10-1500 bp.

Polymerase Chain Reaction (PCR)

The PCR technique has already proven exceptionally valuable in many areas of molecular biology, medicine, and biotechnology. PCR technique has great practical importance and impact on biotechnology. Between 1983 and 1985 American biochemist Kary Mullis developed PCR technique that made it possible to synthesize large quantities of a DNA fragment without cloning it.

Mullis received the 1993 Nobel Prize for Chemistry for his invention. PCR is a cell free amplification technique. Figure 12.31 outlines how PCR technique works. To amplify (make large quantities) a particular DNA sequence by PCR a reaction mixture (often 100μl or less in volume) containing the following are required.
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  • Target DNA
  • Two primers-These are synthetic oligonucleotides, usually about 20 nucleotides long. These are fragments with sequences identical to those flanking the targeted sequence.
  • Thermostable DNA polymerase-Two popular enzymes employed in the PCR technique are Taq polymerase from the thermophilic bacterium.
  • Thermus aquaticus and the vent polymerase from Theromococcus litoralis. These polymerases employed in PCR technique are able to function at high temperatures.
  • Four deoxyribonucleoside triphosphates (dNTPs) – dCTP, dATP, dGTP, dTTP

Steps in PCR

1. Denaturation:

The target DNA containing the sequence to be amplified is heat denatured to separate its complementary strands at temperature 94 °C-95 °C.

2. Annealing:

The temperature is lowered to 37 °C-55 °C so that the primers can hydrogen bond or anneal to the DNA on both sides of the target sequence. Because the primes are present in excess the targeted DNA strands normally anneal to the primers rather than to each other.

3. Extension:

Heat resistant DNA polymerase extends the primers and synthesizes copies of the target DNA sequence using the deoxyribonucleoside triphosphate’s at 70 °C-75 °C.

The three – step cycle (Figure 12.32) is repeated to obtain copies of target DNA in large numbers. At the end of one cylcle, the targeted sequences on both strands have been copied. When the three – step cycle is repeated, the four strands from the first cycle are copied to produce eight fragments.

The third cycle yields 16 products. Theoretically, 20 cycles will Figure 12.32: Three steps PCR cycle The PCR technique has now been automated and is carried out by a specially designed machine (Figure 12.33) PCR machines are now fully automated and microprocessor controlled.
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They can process up to 96 samples at a time. PCR machines can carry out 25 cycles and amplify DNA 105 times in as little as 57 minutes.

The PCR has many applications in research and in commercial arena, including generating specific DNA segments for cloning or sequencing, amplifying DNA to detect specific genetic defects, and amplifying DNA for fingerprinting in crime scene investigation.

PCR technology is improving continually. Various forms of PCR are available. RNA too can be efficiently used in PCR procedures. Cellular RNAs and RNA viruses may be studied even when the RNA is present in very small amounts (as few as 100 copies can be transcribed and amplified). Quantitative PCR is quite valuable in virology and gene impression studies.

PCR is modified as per the specific demands of the situation. Thus there are many variations in the original PCR Examples nested PCR, inverse PCR, reverse transcription PCR, time quantitative PCR, RAPD, RFLP, AFLP.
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